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  • Featured Product
  • KD/KO Validated

Chk1 Polyclonal antibody, PBS Only

Chk1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25887-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 25887-1-AP (same clone as 25887-1-PBS)

    WB analysis using 25887-1-AP (same clone as 25887-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IF Staining of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IP experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IP result of anti-Chk1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHK1 antibody included
  • IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHK1 antibody included
  • FC experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    FC experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

25887-1-PBS targets Chk1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Chk1 fusion protein Ag22993 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CHK1 checkpoint homolog (S. pombe)
Synonyms
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC004202
Gene Symbol Chk1
Gene ID (NCBI) 1111
RRIDAB_2880283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14757
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.