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验证数据展示

  • WB analysis using 25887-1-AP (same clone as 25887-1-PBS)

    WB analysis using 25887-1-AP (same clone as 25887-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IF Staining of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IP experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    IP result of anti-Chk1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHK1 antibody included
  • IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHK1 antibody included
  • FC experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    FC experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Chk1 Polyclonal antibody, PBS Only

Chk1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 25887-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

25887-1-PBS targets Chk1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Chk1 fusion protein Ag22993 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名
计算分子量 54 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
RRIDAB_2880283
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14757
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.