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Chk1 Polyclonal antibody

Chk1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 25887-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

CHEK1, EC:2.7.11.1, CHK1 checkpoint homolog, Chk 1, CHEK 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293T cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25887-1-AP targets Chk1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 Chk1 fusion protein Ag22993 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, EC:2.7.11.1, CHK1 checkpoint homolog, Chk 1, CHEK 1
计算分子量 54 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
RRIDAB_2880283
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

实验方案

Product Specific Protocols
WB protocol for Chk1 antibody 25887-1-APDownload protocol
IHC protocol for Chk1 antibody 25887-1-APDownload protocol
IF protocol for Chk1 antibody 25887-1-APDownload protocol
IP protocol for Chk1 antibody 25887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability

Authors - Zaili Luo
humanWB

Nat Commun

G-quadruplex DNA structures in human stem cells and differentiation.

Authors - Katherine G Zyner
humanWB

Cell Death Differ

Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.

Authors - Yan Zhao
humanWB

Cancer Res

Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.

Authors - Zhe Sun
humanWB

Clin Cancer Res

RNF126 as a biomarker of a poor prognosis in invasive breast cancer and CHK1 inhibitor efficacy in breast cancer cells.

Authors - Xiaosong Yang
humanWB

Haematologica

The HDAC and PI3K dual inhibitor CUDC-907 synergistically enhances the antileukemic activity of venetoclax in preclinical models of acute myeloid leukemia.

Authors - Xinyu Li
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