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Chk1 Recombinant antibody, PBS Only

Chk1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240618E11

Cat no : 84905-1-PBS

Print datasheet

Synonyms

CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1

验证数据展示

  • WB analysis using 84905-1-RR (same clone as 84905-1-PBS)

    WB analysis using 84905-1-RR (same clone as 84905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84905-1-RR (CHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84905-1-RR (same clone as 84905-1-PBS)

    IF Staining of HeLa using 84905-1-RR (same clone as 84905-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHK1 antibody (84905-1-RR, Clone: 240618E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84905-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

84905-1-PBS targets Chk1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Chk1 fusion protein Ag22993 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CHK1 checkpoint homolog (S. pombe)
Synonyms CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC004202
Gene Symbol Chk1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14757
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.