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验证数据展示

  • FC experiment of SH-SY5Y using CL594-60135

    FC experiment of SH-SY5Y using CL594-60135

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Chromogranin A (CL594-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Neuro-2a using CL594-60135

    FC experiment of Neuro-2a using CL594-60135

    1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Chromogranin A (CL594-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Neuro-2a using CL594-60135

    IF Staining of Neuro-2a using CL594-60135

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CoraLite®594 Chromogranin A antibody (CL594-60135, Clone: 3H11C11 ) at dilution of 1:200.

CoraLite®594-conjugated Chromogranin A Monoclonal antibody

Chromogranin A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60135

产品说明书

CloneNo. 3H11C11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

CGA, CHGA, 3H11C11, AL-11, Chromogranin-A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inSH-SY5Y cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60135 targets Chromogranin A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Chromogranin A fusion protein Ag0807 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromogranin A (parathyroid secretory protein 1)
别名 CGA, CHGA, 3H11C11, AL-11, Chromogranin-A
计算分子量 51 kDa
观测分子量 70 kDa
GenBank蛋白编号BC006459
基因名称 Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2923917
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP10645
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
IF protocol for CL594 Chromogranin A antibody CL594-60135Download protocol
FC protocol for CL594 Chromogranin A antibody CL594-60135Download protocol
Standard Protocols
Click here to view our Standard Protocols