验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
82707-13-RR targets Cleaved Caspase 3/P17/P19 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caspase 3, apoptosis-related cysteine peptidase |
| 别名 | CASP3, Caspase3, 230527B2, CASP 3, CASP-3 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 17 kDa, 19 kDa |
| GenBank蛋白编号 | NM_032991 |
| 基因名称 | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_3670535 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P42574 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cleaved Caspase 3/P17/P19 antibody 82707-13-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arch Biochem Biophys DANCR Knockdown Alleviates Neuroinflammation and Functional Recovery after Spinal Cord Injury via Regulating the ACTN4 / STAT3 Axis | ||
CNS Neurosci Ther CHOP-Mediated Disruption of Hippocampal Synaptic Plasticity and Neuronal Activity Contributes to Chronic Pain-Related Cognitive Deficits |