验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
82707-13-RR targets Cleaved Caspase 3 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Synonyms | CASP3, Caspase3, Caspase 3, CASP-3, CASP 3 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 17 kDa, 19 kDa |
| GenBank Accession Number | NM_032991 |
| Gene Symbol | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_3670535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P42574 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cleaved Caspase 3 antibody 82707-13-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |