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Clusterin Recombinant antibody, PBS Only

Clusterin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241277D4

Cat no : 84067-4-PBS

Print datasheet

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

验证数据展示

  • WB analysis of human plasma using 84067-4-RR (same clone as 84067-4-PBS)

    WB analysis of human plasma using 84067-4-RR (same clone as 84067-4-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84067-4-RR (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84067-4-RR (same clone as 84067-4-PBS)

    IF Staining of HeLa using 84067-4-RR (same clone as 84067-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (84067-4-RR, Clone: 241277D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84067-4-RR

    Affinity and Kinetic Characterization of 84067-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human Clusterin were performed. The affinity constant is 6.91 nM.

产品信息

The immunogen of 84067-4-PBS is Clusterin Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name clusterin
Synonyms Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10909
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).