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Clusterin Recombinant antibody

Clusterin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241277D4

Cat no : 84067-4-RR

Print datasheet

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

验证数据展示

  • WB analysis of human plasma using 84067-4-RR

    WB analysis of human plasma using 84067-4-RR

    human plasma were subjected to SDS PAGE followed by western blot with 84067-4-RR (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84067-4-RR

    IF Staining of HeLa using 84067-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (84067-4-RR, Clone: 241277D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84067-4-RR

    Affinity and Kinetic Characterization of 84067-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human Clusterin were performed. The affinity constant is 6.91 nM.

经过测试的应用

Positive WB detected inhuman plasma
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84067-4-RR targets Clusterin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name clusterin
Synonyms Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
WB protocol for Clusterin antibody 84067-4-RRDownload protocol
IF protocol for Clusterin antibody 84067-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols