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验证数据展示

  • IF Staining of HeLa using CL488-84067-4

    IF Staining of HeLa using CL488-84067-4

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Clusterin antibody (CL488-84067-4, Clone: 241277D4 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-84067-4

    FC experiment of HeLa using CL488-84067-4

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Clusterin Recombinant antibody (CL488-84067-4, Clone:241277D4)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Clusterin Recombinant antibody

Clusterin Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84067-4

Print datasheet

CloneNo. 241277D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 84067-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84067-4 targets Clusterin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name clusterin
Synonyms Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
RRIDAB_3673336
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10909
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Clusterin antibody CL488-84067-4Download protocol
FC protocol for CL Plus 488 Clusterin antibody CL488-84067-4Download protocol
Standard Protocols
Click here to view our Standard Protocols