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Collagen Type I Monoclonal antibody, PBS Only

Collagen Type I Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D5E8

Cat no : 66761-1-PBS

Print datasheet

Synonyms

Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4

验证数据展示

  • WB analysis of rat skin using 66761-1-Ig (same clone as 66761-1-PBS)

    WB analysis of rat skin using 66761-1-Ig (same clone as 66761-1-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 66761-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66761-1-PBS in a different storage buffer formulation.

产品信息

66761-1-PBS targets Collagen Type I in WB, Indirect ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, pig, mouse, rat
Immunogen Collagen Type I fusion protein Ag6281 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name collagen, type I, alpha 2
Synonyms Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4
Calculated Molecular Weight 1366 aa, 130 kDa
Observed Molecular Weight 140 kDa, 70-80 kDa
GenBank Accession NumberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
RRIDAB_2882107
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08123
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.