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验证数据展示

  • WB analysis of human placenta using 68652-1-Ig

    WB analysis of human placenta using 68652-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68652-1-Ig (COL6A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 68652-1-Ig

    WB analysis of human heart using 68652-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 68652-1-Ig (COL6A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

COL6A2 Monoclonal antibody

COL6A2 Monoclonal Antibody for WB, ELISA
Cat No. 68652-1-Ig

产品说明书

CloneNo. 3E9C2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, ELISA

别名

3E9C2, Collagen alpha 2(VI) chain, Collagen alpha-2(VI) chain, Collagen Type VI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, human heart tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A2 Ready-To-Use IHC Kit. COL6A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68652-1-Ig targets COL6A2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 COL6A2 fusion protein Ag7134 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type VI, alpha 2
别名 3E9C2, Collagen alpha 2(VI) chain, Collagen alpha-2(VI) chain, Collagen Type VI
计算分子量 109 kDa
观测分子量140 kDa
GenBank蛋白编号BC065509
基因名称 COL6A2
Gene ID (NCBI) 1292
RRIDAB_3670394
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12110
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen VI, composed of three polypeptides, alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains, is a beaded filament collagen found in most connective tissues. The alpha-1(VI) and alpha-2(VI) chains are 140 kDa, while the alpha-3(VI) chain is 200-300 kDa (PMID: 3792302, 3348212). COL6A2 gene encodes the alpha-2 subunit of type VI collagen. The alpha-2(VI) subunit has a calculated molecular weight of 109 kDa, and an apparent molecular weight of 140 kDa due to posttranslational modification. Mutations in COL6A2 gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.

实验方案

Product Specific Protocols
WB protocol for COL6A2 antibody 68652-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols