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Complement factor D Recombinant antibody

Complement factor D Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

242244C2

Cat no : 84851-4-RR

Print datasheet

Synonyms

CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN

验证数据展示

  • WB analysis using 84851-4-RR

    WB analysis using 84851-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84851-4-RR (Complement factor D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 84851-4-RR

    IHC staining of mouse stomach using 84851-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 84851-4-RR

    IHC staining of mouse stomach using 84851-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84851-4-RR

    Affinity and Kinetic Characterization of 84851-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84851-4-RR against Human Complement factor D were performed. The affinity constant is 60.2 pM.

经过测试的应用

Positive WB detected inU-937 cells, THP-1 cells, human placenta tissue
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84851-4-RR targets Complement factor D in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name complement factor D (adipsin)
Synonyms CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN
Calculated Molecular Weight27kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberNM_001928.3
Gene Symbol CFD
Gene ID (NCBI) 1675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00746
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).

实验方案

Product Specific Protocols
WB protocol for Complement factor D antibody 84851-4-RRDownload protocol
IHC protocol for Complement factor D antibody 84851-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols