• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

    WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84851-4-RR (Complement factor D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01660-3

    Sandwich ELISA standard curve of MP01660-3

    Sandwich ELISA standard curve of MP01660-3, Human Complement factor D Recombinant Matched Antibody Pair - PBS only. 84851-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1189. 84851-4-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL.

  • Sample test of MP01660-3

    Sample test of MP01660-3

    Serum of eight individual healthy human donors was measured. The Complement factor D concentration of detected samples was determined to be 838.5 ng/mL with a range of 561.9-1525.4 ng/mL.

  • Affinity and Kinetic Characterization of 84851-4-RR

    Affinity and Kinetic Characterization of 84851-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84851-4-RR against Human Complement factor D were performed. The affinity constant is 60.2 pM.

Complement factor D Recombinant antibody, PBS Only (Detector)

Complement factor D Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84851-4-PBS

产品说明书

CloneNo. 242244C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

别名

CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84851-4-PBS targets Complement factor D as part of a matched antibody pair:

MP01660-3: 84851-2-PBS capture and 84851-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement factor D (adipsin)
别名 CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN
计算分子量27kDa
观测分子量 27 kDa
GenBank蛋白编号NM_001928.3
基因名称 CFD
Gene ID (NCBI) 1675
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00746
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).