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Complement factor D Recombinant antibody, PBS Only (Detector)

Complement factor D Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242244C2

Cat no : 84851-4-PBS

Print datasheet

Synonyms

CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN

验证数据展示

  • WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

    WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84851-4-RR (Complement factor D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01660-3

    Sandwich ELISA standard curve of MP01660-3

    Sandwich ELISA standard curve of MP01660-3, Human Complement factor D Recombinant Matched Antibody Pair - PBS only. 84851-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1189. 84851-4-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL.

  • Sample test of MP01660-3

    Sample test of MP01660-3

    Serum of eight individual healthy human donors was measured. The Complement factor D concentration of detected samples was determined to be 838.5 ng/mL with a range of 561.9-1525.4 ng/mL.

  • Affinity and Kinetic Characterization of 84851-4-RR

    Affinity and Kinetic Characterization of 84851-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84851-4-RR against Human Complement factor D were performed. The affinity constant is 60.2 pM.

产品信息

84851-4-PBS targets Complement factor D as part of a matched antibody pair:

MP01660-3: 84851-2-PBS capture and 84851-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name complement factor D (adipsin)
Synonyms CFD, DF, complement factor D (adipsin), C3 convertase activator, ADIPSIN
Calculated Molecular Weight27kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberNM_001928.3
Gene Symbol CFD
Gene ID (NCBI) 1675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00746
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).