验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
23161-1-AP targets Connexin-50 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Connexin-50 fusion protein Ag19572 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gap junction protein, alpha 8, 50kDa |
| 别名 | CX50, GJA8, GJA 8, Connexin50, Connexin 50 |
| 计算分子量 | 433 aa, 48 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC146507 |
| 基因名称 | GJA8 |
| Gene ID (NCBI) | 2703 |
| RRID | AB_3669410 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P48165 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Connexin-50 (Cx50), also known as Gap junction alpha 8 protein (GJA8), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-50 is expressed in lens fiber cells and is essential for the normal physiological function of the lens (PMID: 11916859). Together with Connexin-46, Connexin-50 forms functional half-gap junction channels in non-junctional membranes. The expected molecular weight of Connexin-50 is 50 kDa, and in some literature, Connexin-50 was detected in lens samples at ~65 kDa (PMID: 31195409; PMID: 29196765).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Connexin-50 antibody 23161-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |