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验证数据展示

  • IF Staining of HepG2 using CL488-26825

    IF Staining of HepG2 using CL488-26825

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CstF-64 antibody (CL488-26825) at dilution of 1:200, CL594-phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated CstF-64 Polyclonal antibody

CstF-64 Polyclonal Antibody for IF/ICC
Cat No. CL488-26825

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CSTF2 Ready-To-Use IHC Kit. CSTF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26825-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26825 targets CstF-64 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CstF-64 fusion protein Ag25254 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
别名 CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64
计算分子量 61 kDa
观测分子量 64 kDa
GenBank蛋白编号BC017712
基因名称 CstF-64
Gene ID (NCBI) 1478
RRIDAB_3672805
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33240
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CstF-64 antibody CL488-26825Download protocol
Standard Protocols
Click here to view our Standard Protocols