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验证数据展示

  • WB analysis using 82148-1-RR (same clone as 82148-1-PBS)

    WB analysis using 82148-1-RR (same clone as 82148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82148-1-RR (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 82148-1-RR (same clone as 82148-1-PBS)

    IHC staining of mouse colon using 82148-1-RR (same clone as 82148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IHC staining of rat colon using 82148-1-RR (same clone as 82148-1-PBS)

    IHC staining of rat colon using 82148-1-RR (same clone as 82148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNA2 antibody included
  • IF Staining of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IF Staining of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin A2 antibody (82148-1-RR, Clone: 1C23 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IP experiment of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

    IP result of anti-Cyclin A2 (IP:82148-1-RR, 4ug; Detection:82148-1-RR 1:3000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82148-1-PBS

    Affinity and Kinetic Characterization of 82148-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82148-1-RR against Human Cyclin A2 were performed. The affinity constant is below 1 pM.

Cyclin A2 Recombinant antibody, PBS Only

Cyclin A2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82148-1-PBS

产品说明书

CloneNo. 1C23

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CCNA2, Cyclin-A, Cyclin A, CCNA, CCN1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.

产品信息

82148-1-PBS targets Cyclin A2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Cyclin A2 fusion protein Ag12765 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin A2
别名 CCNA2, Cyclin-A, Cyclin A, CCNA, CCN1
计算分子量 432 aa, 49 kDa
观测分子量 52 kDa
GenBank蛋白编号BC104783
基因名称 Cyclin A2
Gene ID (NCBI) 890
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20248
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.