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- KD/KO Validated
Cyclin B1 Polyclonal antibody
Cyclin B1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (6)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, HeLa cells, K-562 cells, C6 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human skin cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, HT-29 cells |
Positive FC (Intra) detected in | Ramos cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28603-1-AP targets Cyclin B1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, rat |
Cited Reactivity | human, mouse, rat, pig, canine, chicken, bovine, goat |
Immunogen | Cyclin B1 fusion protein Ag29426 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cyclin B1 |
Synonyms | CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 55-60 kDa |
GenBank Accession Number | BC006510 |
Gene Symbol | Cyclin B1 |
Gene ID (NCBI) | 891 |
RRID | AB_2881179 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P14635 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
IHC protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
IF protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
IP protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Clin Transl Med Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes | ||
Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. | ||
Mol Oncol Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma | ||
Life Sci Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway | ||
Am J Physiol Lung Cell Mol Physiol KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension |