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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-28603

Print datasheet

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

验证数据展示

  • IF Staining of HT-29 using CL488-28603

    IF Staining of HT-29 using CL488-28603

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-28603) at dilution of 1:200.

  • IF Staining of HeLa using CL488-28603

    IF Staining of HeLa using CL488-28603

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-28603) at dilution of 1:200.

经过测试的应用

Positive IF detected inHT-29 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28603-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28603 targets Cyclin B1 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen Cyclin B1 fusion protein Ag29426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin B1
Synonyms CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC006510
Gene Symbol CCNB1
Gene ID (NCBI) 891
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14635
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin B1 antibody CL488-28603Download protocol
Standard Protocols
Click here to view our Standard Protocols