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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67726-1-Ig (same clone as 67726-1-PBS)

    WB analysis using 67726-1-Ig (same clone as 67726-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67726-1-Ig (same clone as 67726-1-PBS)

    WB analysis of HeLa using 67726-1-Ig (same clone as 67726-1-PBS)

    WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells. This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

    IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNB2 antibody included
  • IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

    IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNB2 antibody included
  • IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

    IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

    IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 67726-1-Ig (same clone as 67726-1-PBS)

    IF Staining of PC-12 using 67726-1-Ig (same clone as 67726-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Cyclin B2 Monoclonal antibody, PBS Only

Cyclin B2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67726-1-PBS

产品说明书

CloneNo. 2G8B7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CCNB2 Ready-To-Use IHC Kit. CCNB2 primary antibody included.

产品信息

67726-1-PBS targets Cyclin B2 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Cyclin B2 fusion protein Ag16329 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin B2
别名 CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7
计算分子量 398 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC105086
基因名称 Cyclin B2
Gene ID (NCBI) 9133
RRIDAB_2882912
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95067
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.