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  • Featured Product
  • KD/KO Validated

Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2G8B7

Cat no : 67726-1-Ig

Print datasheet

Synonyms

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

验证数据展示

  • WB analysis using 67726-1-Ig

    WB analysis using 67726-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67726-1-Ig

    WB analysis of HeLa using 67726-1-Ig

    WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells.

  • IHC staining of human breast cancer using 67726-1-Ig

    IHC staining of human breast cancer using 67726-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCNB2 antibody included
  • IHC staining of human breast cancer using 67726-1-Ig

    IHC staining of human breast cancer using 67726-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCNB2 antibody included
  • IF Staining of mouse testis using 67726-1-Ig

    IF Staining of mouse testis using 67726-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse testis using 67726-1-Ig

    IF Staining of mouse testis using 67726-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of PC-12 using 67726-1-Ig

    IF Staining of PC-12 using 67726-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells, K-562 cells, Jurkat cells, HEK-293 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy CCNB2 Ready-To-Use IHC Kit. CCNB2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67726-1-Ig targets Cyclin B2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Cyclin B2 fusion protein Ag16329 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin B2
Synonyms CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7
Calculated Molecular Weight 398 aa, 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC105086
Gene Symbol Cyclin B2
Gene ID (NCBI) 9133
RRIDAB_2882912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95067
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.

实验方案

Product Specific Protocols
WB protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
IHC protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
IF protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols