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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G8B7

Cat no : CL647-67726

Print datasheet

Synonyms

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

验证数据展示

  • IF Staining of mouse testis using CL647-67726

    IF Staining of mouse testis using CL647-67726

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 Cyclin B2 antibody (CL647-67726, Clone: 2G8B7 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy CCNB2 Ready-To-Use IHC Kit. CCNB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67726-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67726 targets Cyclin B2 in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen Cyclin B2 fusion protein Ag16329 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin B2
Synonyms CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7
Calculated Molecular Weight 398 aa, 45 kDa
GenBank Accession NumberBC105086
Gene Symbol Cyclin B2
Gene ID (NCBI) 9133
RRIDAB_2920301
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95067
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Cyclin B2 antibody CL647-67726Download protocol
Standard Protocols
Click here to view our Standard Protocols