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验证数据展示

  • IF Staining of mouse testis using CL647-67726

    IF Staining of mouse testis using CL647-67726

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 Cyclin B2 antibody (CL647-67726, Clone: 2G8B7 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 647-conjugated Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for IF-P
Cat No. CL647-67726

产品说明书

CloneNo. 2G8B7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF-P

别名

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy CCNB2 Ready-To-Use IHC Kit. CCNB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67726-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67726 targets Cyclin B2 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 Cyclin B2 fusion protein Ag16329 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin B2
别名 CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7
计算分子量 398 aa, 45 kDa
GenBank蛋白编号BC105086
基因名称 Cyclin B2
Gene ID (NCBI) 9133
RRIDAB_2920301
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95067
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Cyclin B2 antibody CL647-67726Download protocol
Standard Protocols
Click here to view our Standard Protocols