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CoraLite® Plus 488-conjugated Cyclin D3 Monoclonal antibody

Cyclin D3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66357

产品说明书

CloneNo. 1F2C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

CCND3, G1/S-specific cyclin-D3, G1/S specific cyclin D3, Cyclin D, 1F2C6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CCND3 Ready-To-Use IHC Kit. CCND3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66357-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66357 targets Cyclin D3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Cyclin D3 fusion protein Ag22825 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin D3
别名 CCND3, G1/S-specific cyclin-D3, G1/S specific cyclin D3, Cyclin D, 1F2C6
计算分子量 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC011616
基因名称 Cyclin D3
Gene ID (NCBI) 896
ENSEMBL Gene IDENSG00000112576
RRIDAB_3084236
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30281
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin D3 antibody CL488-66357Download protocol
FC protocol for CL Plus 488 Cyclin D3 antibody CL488-66357Download protocol
Standard Protocols
Click here to view our Standard Protocols
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