验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66357 targets Cyclin D3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | Cyclin D3 fusion protein Ag22825 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | cyclin D3 |
别名 | CCND3, G1/S-specific cyclin-D3, G1/S specific cyclin D3, Cyclin D, 1F2C6 |
计算分子量 | 33 kDa |
观测分子量 | 33-35 kDa |
GenBank蛋白编号 | BC011616 |
基因名称 | Cyclin D3 |
Gene ID (NCBI) | 896 |
ENSEMBL Gene ID | ENSG00000112576 |
RRID | AB_3084236 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P30281 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Cyclin D3 antibody CL488-66357 | Download protocol |
FC protocol for CL Plus 488 Cyclin D3 antibody CL488-66357 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |