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Cyclin E Recombinant antibody

Cyclin E Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240136G9

Cat No : 82700-8-RR

Print datasheet

Synonyms

CCNE, CCNE1, cyclin E1, G1/S specific cyclin E1

验证数据展示

  • IF Staining of HepG2 using 82700-8-RR

    IF Staining of HepG2 using 82700-8-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity experiments of 82700-8-RR

    Affinity experiments of 82700-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-8-RR against Human Cyclin E were performed. The affinity constant is 0.229 nM.

经过测试的应用

Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 82700-8-RR is Cyclin E Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Cyclin E fusion protein Ag2110 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin E1
Synonyms CCNE, CCNE1, cyclin E1, G1/S specific cyclin E1
Calculated Molecular Weight 410 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC035498
Gene Symbol CCNE1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

实验方案

Product Specific Protocols
IF protocol for Cyclin E antibody 82700-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols