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Cyclin G Polyclonal antibody

Cyclin G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29306-1-AP

Print datasheet

Synonyms

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

验证数据展示

  • WB analysis using 29306-1-AP

    WB analysis using 29306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29306-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMDA-MB-231 cells, MDA-MB-453s cells, T-47D cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29306-1-AP targets Cyclin G in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Cyclin G fusion protein Ag30937 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin G1
Synonyms CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC007093
Gene Symbol Cyclin G
Gene ID (NCBI) 900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51959
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin G is also named as CCNG, CYCG1 and CCNG1, and and belongs to the cyclin family. Cyclin G may play a role in growth regulation. Cyclin G is associated with G2/M phase arrest in response to DNA damage. And it may be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation, and levels of Cyclin G are increased after induction of p53 by DNA damage (PMID:12015958). Cyclin G is high levels in skeletal muscle, ovary, kidney and colon. Cyclin G is very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells, consistent high levels of Cyclin G are found throughout the cell cycle (PMID:11063931).

实验方案

Product Specific Protocols
WB protocol for Cyclin G antibody 29306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols