验证数据展示
经过测试的应用
Positive WB detected in | MDA-MB-231 cells, MDA-MB-453s cells, T-47D cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
29306-1-AP targets Cyclin G in WB, IF, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | Human |
文献引用反应性 | mouse |
免疫原 | Cyclin G fusion protein Ag30937 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cyclin G1 |
别名 | CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1 |
计算分子量 | 34 kDa |
观测分子量 | 34 kDa |
GenBank蛋白编号 | BC007093 |
基因名称 | Cyclin G |
Gene ID (NCBI) | 900 |
RRID | AB_3086111 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P51959 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin G is also named as CCNG, CYCG1 and CCNG1, and and belongs to the cyclin family. Cyclin G may play a role in growth regulation. Cyclin G is associated with G2/M phase arrest in response to DNA damage. And it may be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation, and levels of Cyclin G are increased after induction of p53 by DNA damage (PMID:12015958). Cyclin G is high levels in skeletal muscle, ovary, kidney and colon. Cyclin G is very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells, consistent high levels of Cyclin G are found throughout the cell cycle (PMID:11063931).
实验方案
Product Specific Protocols | |
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WB protocol for Cyclin G antibody 29306-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |