验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, MDA-MB-453s cells, T-47D cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
29306-1-AP targets Cyclin G in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Immunogen | Cyclin G fusion protein Ag30937 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin G1 |
| Synonyms | CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC007093 |
| Gene Symbol | Cyclin G |
| Gene ID (NCBI) | 900 |
| RRID | AB_3086111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51959 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin G is also named as CCNG, CYCG1 and CCNG1, and and belongs to the cyclin family. Cyclin G may play a role in growth regulation. Cyclin G is associated with G2/M phase arrest in response to DNA damage. And it may be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation, and levels of Cyclin G are increased after induction of p53 by DNA damage (PMID:12015958). Cyclin G is high levels in skeletal muscle, ovary, kidney and colon. Cyclin G is very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells, consistent high levels of Cyclin G are found throughout the cell cycle (PMID:11063931).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin G antibody 29306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |