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  • Featured Product
  • KD/KO Validated

Cyclophilin A Polyclonal antibody, PBS Only

Cyclophilin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10720-1-PBS

Print datasheet

Synonyms

PPIA, Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, CYPH, CYPA

验证数据展示

  • WB analysis using 10720-1-AP (same clone as 10720-1-PBS)

    WB analysis using 10720-1-AP (same clone as 10720-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

    IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIA antibody included
  • IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

    IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIA antibody included
  • IF Staining of HepG2 using 10720-1-AP (same clone as 10720-1-PBS)

    IF Staining of HepG2 using 10720-1-AP (same clone as 10720-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cyclophilin A antibody (10720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10720-1-AP (same clone as 10720-1-PBS)

    FC experiment of HeLa using 10720-1-AP (same clone as 10720-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclophilin A Polyclonal antibody (10720-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.

产品信息

10720-1-PBS targets Cyclophilin A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cyclophilin A fusion protein Ag1120 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase A (cyclophilin A)
Synonyms PPIA, Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, CYPH, CYPA
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC005320
Gene Symbol PPIA
Gene ID (NCBI) 5478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62937
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.