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验证数据展示

  • WB analysis using CL488-66047

    WB analysis using CL488-66047

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66047 (Cyclophilin B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cyclophilin B Monoclonal antibody

Cyclophilin B Monoclonal Antibody for WB
Cat No. CL488-66047

产品说明书

CloneNo. 8H4F1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB

别名

PPIB, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1, CYPB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PPIB Ready-To-Use IHC Kit. PPIB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66047-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66047 targets Cyclophilin B in WB applications and shows reactivity with human, mouse samples.

经测试应用 WB Application Description
经测试反应性 human, mouse
免疫原 Cyclophilin B fusion protein Ag17957 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl isomerase B (cyclophilin B)
别名 PPIB, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1, CYPB
计算分子量 216 aa, 24 kDa
观测分子量 19-24 kDa
GenBank蛋白编号BC032138
基因名称 Cyclophilin B
Gene ID (NCBI) 5479
RRIDAB_3084200
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23284
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Cyclophilin B antibody CL488-66047Download protocol
Standard Protocols
Click here to view our Standard Protocols