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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cystatin C Polyclonal antibody

Cystatin C Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12245

Print datasheet

Synonyms

CST3, Post-gamma-globulin, Gamma-trace, Cystatin-C, Cystatin-3

验证数据展示

  • IF Staining of mouse kidney using CL488-12245

    IF Staining of mouse kidney using CL488-12245

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using CL488-12245

    IF Staining of HeLa using CL488-12245

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12245-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12245 targets Cystatin C in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse
Immunogen Cystatin C fusion protein Ag2890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cystatin C
Synonyms CST3, Post-gamma-globulin, Gamma-trace, Cystatin-C, Cystatin-3
Calculated Molecular Weight 146 aa, 16 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC013083
Gene Symbol Cystatin C
Gene ID (NCBI) 1471
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01034
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin C antibody CL488-12245Download protocol
Standard Protocols
Click here to view our Standard Protocols