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验证数据展示

  • IF Staining of mouse kidney using CL488-12245

    IF Staining of mouse kidney using CL488-12245

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using CL488-12245

    IF Staining of HeLa using CL488-12245

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cystatin C antibody (CL488-12245) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cystatin C Polyclonal antibody

Cystatin C Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL488-12245

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, IF-P

别名

CST3, Post-gamma-globulin, Gamma-trace, Cystatin-C, Cystatin-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12245-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12245 targets Cystatin C in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse
免疫原 Cystatin C fusion protein Ag2890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cystatin C
别名 CST3, Post-gamma-globulin, Gamma-trace, Cystatin-C, Cystatin-3
计算分子量 146 aa, 16 kDa
观测分子量 13 kDa
GenBank蛋白编号BC013083
基因名称 Cystatin C
Gene ID (NCBI) 1471
RRIDAB_3083922
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01034
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin C antibody CL488-12245Download protocol
Standard Protocols
Click here to view our Standard Protocols