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验证数据展示

  • WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

Cytoglobin Monoclonal antibody, PBS Only

Cytoglobin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60228-1-PBS

产品说明书

CloneNo. 4C7E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CYGB, cytoglobin, HGB, Histoglobin, STAP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60228-1-PBS targets Cytoglobin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, pig, rat
免疫原 Cytoglobin fusion protein Ag4161 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytoglobin
别名 CYGB, cytoglobin, HGB, Histoglobin, STAP
计算分子量 190 aa, 21 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC029798
基因名称 Cytoglobin
Gene ID (NCBI) 114757
RRIDAB_11182383
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WWM9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.