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Cytoglobin Monoclonal antibody, PBS Only

Cytoglobin Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

4C7E11

Cat No : 60228-1-PBS

Print datasheet

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

验证数据展示

  • WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

产品信息

60228-1-PBS targets Cytoglobin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen Cytoglobin fusion protein Ag4161 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoglobin
Synonyms CYGB, cytoglobin, HGB, Histoglobin, STAP
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC029798
Gene Symbol CYGB
Gene ID (NCBI) 114757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WWM9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.