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Cytoglobin Monoclonal antibody, PBS Only

Cytoglobin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4C7E11

Cat no : 60228-1-PBS

Print datasheet

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

验证数据展示

  • WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    WB analysis of human heart using 60228-1-Ig (same clone as 60228-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    WB analysis using 60228-1-Ig (same clone as 60228-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    IHC staining of human liver using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    IF Staining of HeLa using 60228-1-Ig (same clone as 60228-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60228-1-PBS in a different storage buffer formulation.

产品信息

60228-1-PBS targets Cytoglobin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen Cytoglobin fusion protein Ag4161 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoglobin
Synonyms CYGB, cytoglobin, HGB, Histoglobin, STAP
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC029798
Gene Symbol Cytoglobin
Gene ID (NCBI) 114757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WWM9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.