Cytoglobin Monoclonal antibody
Cytoglobin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4C7E11
验证数据展示
经过测试的应用
| Positive WB detected in | Human heart, pig heart tissue, human heart tissue, Transfected HEK-293 cells, rabbit heart tissue |
| Positive IHC detected in | human liver tissue, human heart tissue, human bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
60228-1-Ig targets Cytoglobin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, pig, rat |
| Cited Reactivity | human |
| Immunogen | Cytoglobin fusion protein Ag4161 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytoglobin |
| Synonyms | CYGB, cytoglobin, HGB, Histoglobin, STAP |
| Calculated Molecular Weight | 190 aa, 21 kDa |
| Observed Molecular Weight | 27-29 kDa |
| GenBank Accession Number | BC029798 |
| Gene Symbol | Cytoglobin |
| Gene ID (NCBI) | 114757 |
| RRID | AB_11182383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WWM9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| IHC protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| IF protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells. | ||
Cell Death Dis ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma. |
