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CoraLite® Plus 488-conjugated Cytoglobin Monoclonal antibody

Cytoglobin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C7E11

Cat no : CL488-60228

Print datasheet

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

验证数据展示

  • IF Staining of HeLa using CL488-60228

    IF Staining of HeLa using CL488-60228

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-60228 (Cytoglobin antibody) at dilution of 1:100. .

  • IF Staining of HeLa using CL488-60228

    IF Staining of HeLa using CL488-60228

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytoglobin antibody (CL488-60228, Clone: 4C7E11 ) at dilution of 1:200.

  • IF Staining of HeLa using CL488-60228

    IF Staining of HeLa using CL488-60228

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytoglobin antibody (CL488-60228, Clone: 4C7E11 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60228-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60228 targets Cytoglobin in IF/ICC applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen Cytoglobin fusion protein Ag4161 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoglobin
Synonyms CYGB, cytoglobin, HGB, Histoglobin, STAP
Calculated Molecular Weight 190 aa, 21 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC029798
Gene Symbol Cytoglobin
Gene ID (NCBI) 114757
RRIDAB_2883122
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WWM9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytoglobin antibody CL488-60228Download protocol
Standard Protocols
Click here to view our Standard Protocols