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Cytokeratin 13 Monoclonal antibody, PBS Only

Cytokeratin 13 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10G2

Cat No : 66684-1-PBS

Print datasheet

Synonyms

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13

验证数据展示

  • WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

产品信息

66684-1-PBS targets Cytokeratin 13 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 13
Synonyms CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002661
Gene Symbol KRT13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13646
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.