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验证数据展示

  • WB analysis of A431 using 66684-1-Ig

    WB analysis of A431 using 66684-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 66684-1-Ig

    IHC staining of human cervical cancer using 66684-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human cervical cancer using 66684-1-Ig

    IHC staining of human cervical cancer using 66684-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • IF Staining of human cervical cancer using 66684-1-Ig

    IF Staining of human cervical cancer using 66684-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of A431 using 66684-1-Ig

    WB analysis of A431 using 66684-1-Ig

    WB result of Cytokeratin 13 antibody (66684-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

  • IHC staining of human cervical cancer using 66684-1-Ig

    IHC staining of human cervical cancer using 66684-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • Featured Product
  • KD/KO验证

Cytokeratin 13 Monoclonal antibody

Cytokeratin 13 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66684-1-Ig

产品说明书

CloneNo. 1E10G2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, rat skin tissue
Positive IHC detected inhuman cervical cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman cervical cancer tissue
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66684-1-Ig targets Cytokeratin 13 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原 Cytokeratin 13 fusion protein Ag0217 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 13
别名 KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002661
基因名称 Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2882038
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13646
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
IHC protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
IF protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols