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验证数据展示

  • WB analysis using CL488-66802

    WB analysis using CL488-66802

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66802 (Cytokeratin 16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cytokeratin 16 Monoclonal antibody

Cytokeratin 16 Monoclonal Antibody for WB
Cat No. CL488-66802

产品说明书

CloneNo. 2H4D8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT16 Ready-To-Use IHC Kit. KRT16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66802-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66802 targets Cytokeratin 16 in WB applications and shows reactivity with human samples.

经测试应用 WB Application Description
经测试反应性 human
免疫原 Cytokeratin 16 fusion protein Ag11240 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 16
别名
计算分子量 473 aa, 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC039169
基因名称 Cytokeratin 16
Gene ID (NCBI) 3868
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08779
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Cytokeratin 16 antibody CL488-66802Download protocol
Standard Protocols
Click here to view our Standard Protocols