• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 16 Monoclonal antibody

Cytokeratin 16 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H4D8

Cat no : CL488-66802

Print datasheet

Synonyms

验证数据展示

  • WB analysis using CL488-66802

    WB analysis using CL488-66802

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66802 (Cytokeratin 16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT16 Ready-To-Use IHC Kit. KRT16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66802-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66802 targets Cytokeratin 16 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen Cytokeratin 16 fusion protein Ag11240 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 16
Synonyms
Calculated Molecular Weight 473 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC039169
Gene Symbol Cytokeratin 16
Gene ID (NCBI) 3868
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08779
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Cytokeratin 16 antibody CL488-66802Download protocol
Standard Protocols
Click here to view our Standard Protocols