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Cytokeratin 18 Monoclonal antibody, PBS Only (Detector)

Cytokeratin 18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat no : 66187-1-PBS

Print datasheet

Synonyms

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

验证数据展示

  • WB analysis using 66187-1-Ig (same clone as 66187-1-PBS)

    WB analysis using 66187-1-Ig (same clone as 66187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66187-1-Ig (same clone as 66187-1-PBS)

    IF Staining of A549 using 66187-1-Ig (same clone as 66187-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

    IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

    IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IF Staining of mouse testis using 66187-1-Ig (same clone as 66187-1-PBS)

    IF Staining of mouse testis using 66187-1-Ig (same clone as 66187-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

    FC experiment of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 18 (66187-1-Ig, Clone:1G11C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50238-1

    Cytometric bead array standard curve of MP50238-1

    Cytometric bead array standard curve of MP50238-1, KRT18 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66187-2-PBS. Detection antibody: 66187-1-PBS. Standard:Ag1260. Range: 0.391-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

产品信息

66187-1-PBS targets Cytokeratin 18 as part of a matched antibody pair:

MP50238-1: 66187-2-PBS capture and 66187-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Cytokeratin 18 fusion protein Ag1260 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 18
Synonyms KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000180
Gene Symbol Cytokeratin 18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05783
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.