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验证数据展示

  • WB analysis using 66187-1-Ig

    WB analysis using 66187-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 66187-1-Ig

    IF Staining of A549 using 66187-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human kidney using 66187-1-Ig

    IHC staining of human kidney using 66187-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human kidney using 66187-1-Ig

    IHC staining of human kidney using 66187-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human liver using 66187-1-Ig

    IHC staining of human liver using 66187-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT18 antibody included
  • IF Staining of mouse testis using 66187-1-Ig

    IF Staining of mouse testis using 66187-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66187-1-Ig

    FC experiment of HeLa using 66187-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 18 (66187-1-Ig, Clone:1G11C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66187-1-Ig

产品说明书

CloneNo. 1G11C4
引用文献(18)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

别名

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, pig skin tissue, A431 cells, HeLa cells, HepG2 cells, COLO 320 cells, K-562 cells, 4T1 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue, human colon cancer tissue, human liver tissue, human liver cancer tissue, human lung cancer tissue, human renal cell carcinoma tissue, human tonsillitis tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inA549 cells, HUVEC cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:600-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66187-1-Ig targets Cytokeratin 18 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse
免疫原 Cytokeratin 18 fusion protein Ag1260 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 18
别名 KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000180
基因名称 Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2881582
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05783
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
IHC protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
IF protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Cell Biol

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties

Authors - Dennis K Jeppesen
View Article
mouseIF

Redox Biol

ACSL4 deficiency confers protection against ferroptosis-mediated acute kidney injury.

Authors - Yue Wang
View Article
humanIF

J Pathol

Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2.

Authors - Qiuli Liu
View Article
humanWB

Hum Reprod

T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis.

Authors - Qinsheng Lu
View Article
mouseIF

Stem Cell Res Ther

Expansion of mouse castration-resistant intermediate prostate stem cells in vitro

Authors - Yalan Xu
View Article
humanIF,IHC

Commun Biol

Single-cell analysis supports a luminal-neuroendocrine transdifferentiation in human prostate cancer.

Authors - Baijun Dong
View Article