FITC-conjugated Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. FITC-66187

产品说明书

CloneNo. 1G11C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig and More (1)

应用

IF/ICC, FC (Intra) and More (1)

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-66187 targets Cytokeratin 18 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, bovine
免疫原 Cytokeratin 18 fusion protein Ag1260 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 18
别名 CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000180
基因名称 Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2920504
偶联类型 FITC Fluorescent Dye
最大激发/发射波长498 nm / 526 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05783
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

实验方案

Product Specific Protocols
IF protocol for FITC Cytokeratin 18 antibody FITC-66187Download protocol
FC protocol for FITC Cytokeratin 18 antibody FITC-66187Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineFC

J Dairy Sci

Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver

Authors - Wei Yang
bovineIF

J Dairy Sci

Diacylglycerol O-acyltransferase (DGAT) isoforms play a role in peridroplet mitochondrial fatty acid metabolism in bovine liver

Authors - Shuang Wang
humanIF

Exp Ther Med

Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer

Authors - Jianping Jiang
bovineIF

J Dairy Sci

Caveolin 1 in bovine liver is associated with fatty acid-induced lipid accumulation and the ER unfolded protein response: role in fatty liver development

Authors - Ming Li
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