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验证数据展示

  • IF Staining of HeLa using CL532-66187

    IF Staining of HeLa using CL532-66187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 532 Cytokeratin 18 antibody (CL532-66187, Clone: 1G11C4 ) at dilution of 1:200.

CoraLite® Plus 532-conjugated Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for IF/ICC
Cat No. CL532-66187

Print datasheet

CloneNo. 1G11C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 532
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66187-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-66187 targets Cytokeratin 18 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 18 fusion protein Ag1260 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 18
Synonyms CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000180
Gene Symbol Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2934632
Conjugate CoraLite® Plus 532 Fluorescent Dye
Excitation/Emission Maxima Wavelengths537 nm / 560 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05783
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

实验方案

Product Specific Protocols
IF protocol for CL Plus 532 Cytokeratin 18 antibody CL532-66187Download protocol
Standard Protocols
Click here to view our Standard Protocols