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Cytokeratin 18 Recombinant antibody, PBS Only

Cytokeratin 18 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F2

Cat no : 82817-5-PBS

Print datasheet

Synonyms

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

验证数据展示

  • WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

    WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of Human bowens disease using 82817-5-RR (same clone as 82817-5-PBS)

    IHC staining of Human bowens disease using 82817-5-RR (same clone as 82817-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

    IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (82817-5-RR, Clone: 4F2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

产品信息

82817-5-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 18 fusion protein Ag1260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 18
Synonyms KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC000180
Gene Symbol Cytokeratin 18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05783
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.