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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

    WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT18 antibody included
  • IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

    IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (82817-5-RR, Clone: 4F2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

Cytokeratin 18 Recombinant antibody, PBS Only

Cytokeratin 18 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82817-5-PBS

产品说明书

CloneNo. 4F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

产品信息

82817-5-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 18 fusion protein Ag1260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 18
别名 KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC000180
基因名称 Cytokeratin 18
Gene ID (NCBI) 3875
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05783
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.