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验证数据展示

  • WB analysis using 82817-5-RR

    WB analysis using 82817-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 82817-5-RR

    IHC staining of human appendicitis using 82817-5-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT18 antibody included
  • IHC staining of human appendicitis using 82817-5-RR

    IHC staining of human appendicitis using 82817-5-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT18 antibody included
  • IF Staining of HepG2 using 82817-5-RR

    IF Staining of HepG2 using 82817-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (82817-5-RR, Clone: 4F2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Cytokeratin 18 Recombinant antibody

Cytokeratin 18 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82817-5-RR

产品说明书

CloneNo. 4F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A549 cells, HCT 116 cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman appendicitis tissue, human breast cancer tissue, human liver tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82817-5-RR targets Cytokeratin 18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 18 fusion protein Ag1260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 18
别名 KRT18, Cytokeratin,Keratin, CK-18, CK18, CK 18
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC000180
基因名称 Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_3086546
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05783
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 82817-5-RRDownload protocol
IHC protocol for Cytokeratin 18 antibody 82817-5-RRDownload protocol
IF protocol for Cytokeratin 18 antibody 82817-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols