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Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29855-1-AP

Print datasheet

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

验证数据展示

  • WB analysis using 29855-1-AP

    WB analysis using 29855-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 29855-1-AP

    IHC staining of human breast cancer using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT19 antibody included
  • IHC staining of human breast cancer using 29855-1-AP

    IHC staining of human breast cancer using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT19 antibody included
  • IHC staining of human normal colon using 29855-1-AP

    IHC staining of human normal colon using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT19 antibody included
  • IF Staining of HepG2 using 29855-1-AP

    IF Staining of HepG2 using 29855-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells, HT-29 cells, HepG2 cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human thyroid cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29855-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Cytokeratin 19 fusion protein Ag31469 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 19
Synonyms CK19, CK 19, KRT19, K19, Cytokeratin-19
Calculated Molecular Weight44KD
Observed Molecular Weight40-44 kDa
GenBank Accession NumberNM_002276
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols