IHCeasy KRT19 Ready-To-Use IHC Kit
KRT19 Ready-to-use reagent kit for IHC.
Reactivity
Human, Rat
Sample Type
FFPE tissue
Cat No : KHC0033
Synonyms
CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19
Validation Data Gallery
Product Information
KHC0033 is a ready-to-use IHC kit for staining of Cytokeratin 19(CK19 or KRT19). The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy KRT19 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19 |