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Cytokeratin 5/6 Monoclonal antibody

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F9E1

Cat no : 68295-1-Ig

Print datasheet

Synonyms

KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6

验证数据展示

  • WB analysis using 68295-1-Ig

    WB analysis using 68295-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 68295-1-Ig

    IHC staining of human cervical cancer using 68295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IHC staining of human cervical cancer using 68295-1-Ig

    IHC staining of human cervical cancer using 68295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IF Staining of human cervical cancer using 68295-1-Ig

    IF Staining of human cervical cancer using 68295-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 68295-1-Ig

    IF Staining of HeLa using 68295-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman cervical cancer tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1150-1:4600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68295-1-Ig targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 5/6 fusion protein Ag11288 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 6B
Synonyms KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6
Calculated Molecular Weight 564 aa, 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 5/6 antibody 68295-1-IgDownload protocol
IHC protocol for Cytokeratin 5/6 antibody 68295-1-IgDownload protocol
IF protocol for Cytokeratin 5/6 antibody 68295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols