验证数据展示
经过测试的应用
Positive WB detected in | HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue |
Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human cervical cancer tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1150-1:4600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68295-1-Ig targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Cytokeratin 5/6 fusion protein Ag11288 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | keratin 6B |
Synonyms | KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6 |
Calculated Molecular Weight | 564 aa, 60 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC034535 |
Gene Symbol | Cytokeratin 6B |
Gene ID (NCBI) | 3854 |
RRID | AB_2935375 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P04259 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
IHC protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
IF protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |