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Cytokeratin 5/6 Monoclonal antibody, PBS Only

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9E1

Cat no : 68295-1-PBS

Print datasheet

Synonyms

KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6

验证数据展示

  • WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

    WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

    IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.

产品信息

68295-1-PBS targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 5/6 fusion protein Ag11288 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 6B
Synonyms KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6
Calculated Molecular Weight 564 aa, 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04259
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.