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验证数据展示

  • IF Staining of A431 using CL594-28506

    IF Staining of A431 using CL594-28506

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 Cytokeratin 5 antibody (CL594-28506) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-28506

    IF Staining of human tonsillitis using CL594-28506

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite®594 Cytokeratin 5 antibody (CL594-28506) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite®594-conjugated Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL594-28506

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

CK 5, CK-5, keratin 5, KRT5, Cytokeratin5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 28506-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-28506 targets Cytokeratin 5 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 5 fusion protein Ag25436 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 5
别名 CK 5, CK-5, keratin 5, KRT5, Cytokeratin5
计算分子量 590 aa, 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC024292
基因名称 Cytokeratin 5
Gene ID (NCBI) 3852
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13647
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytokeratin 5 antibody CL594-28506Download protocol
Standard Protocols
Click here to view our Standard Protocols