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验证数据展示

  • IF Staining of A431 using CL594-28506

    IF Staining of A431 using CL594-28506

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 Cytokeratin 5 antibody (CL594-28506) at dilution of 1:200.

CoraLite®594-conjugated Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for IF/ICC
Cat No. CL594-28506

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 28506-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-28506 targets Cytokeratin 5 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 5 fusion protein Ag25436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 5
Synonyms
Calculated Molecular Weight 590 aa, 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC024292
Gene Symbol Cytokeratin 5
Gene ID (NCBI) 3852
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13647
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytokeratin 5 antibody CL594-28506Download protocol
Standard Protocols
Click here to view our Standard Protocols