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验证数据展示

  • IF Staining of human oesophagus cancer using CL594-66727

    IF Staining of human oesophagus cancer using CL594-66727

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®594 Cytokeratin 5 antibody (CL594-66727, Clone: 1A1C5 ) at dilution of 1:200.

  • IF Staining of human oesophagus cancer using CL594-66727

    IF Staining of human oesophagus cancer using CL594-66727

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®594 Cytokeratin 5 antibody (CL594-66727, Clone: 1A1C5 ) at dilution of 1:200.

CoraLite®594-conjugated Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for IF-P
Cat No. CL594-66727

产品说明书

CloneNo. 1A1C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig

应用

IF-P

别名

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman oesophagus cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66727-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66727 targets Cytokeratin 5 in IF-P applications and shows reactivity with Human, mouse, pig samples.

经测试应用 IF-P Application Description
经测试反应性 Human, mouse, pig
免疫原 Cytokeratin 5 fusion protein Ag24184 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 5
别名 58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5
计算分子量 590 aa, 62 kDa
GenBank蛋白编号BC024292
基因名称 Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2920028
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13647
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytokeratin 5 antibody CL594-66727Download protocol
Standard Protocols
Click here to view our Standard Protocols