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CoraLite® Plus 647-conjugated Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10590

Print datasheet

Synonyms

CK 6A, CK 6D, CK6A, CK6C, CK6D, Cytokeratin 6A, Cytokeratin 6D, Hom s 5, K6A, K6C, K6D, keratin 6A, KRT conserve, KRT6, KRT6A, KRT6C, KRT6D, Type II keratin Kb6

验证数据展示

  • IF Staining of A431 using CL647-10590

    IF Staining of A431 using CL647-10590

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 647 Cytokeratin 6A antibody (CL647-10590) at dilution of 1:200.

  • FC experiment of A431 using CL647-10590

    FC experiment of A431 using CL647-10590

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Cytokeratin 6A (CL647-10590) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT6 Ready-To-Use IHC Kit. KRT6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10590-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10590 targets Cytokeratin 6A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 6A fusion protein Ag0882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 6A
Synonyms CK 6A, CK 6D, CK6A, CK6C, CK6D, Cytokeratin 6A, Cytokeratin 6D, Hom s 5, K6A, K6C, K6D, keratin 6A, KRT conserve, KRT6, KRT6A, KRT6C, KRT6D, Type II keratin Kb6
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC008807
Gene Symbol Cytokeratin 6A
Gene ID (NCBI) 3853
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02538
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Cytokeratin 6A antibody CL647-10590Download protocol
FC protocol for CL Plus 647 Cytokeratin 6A antibody CL647-10590Download protocol
Standard Protocols
Click here to view our Standard Protocols