• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Cytokeratin 6B Polyclonal antibody, PBS Only

Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 17391-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

    WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.

产品信息

17391-1-PBS targets Cytokeratin 6B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 6B fusion protein Ag11288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 6B
Synonyms
Calculated Molecular Weight 564 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
RRIDAB_2134318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04259
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.