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验证数据展示

  • WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

    WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Cytokeratin 6B Polyclonal antibody, PBS Only

Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 17391-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.

产品信息

17391-1-PBS targets Cytokeratin 6B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 6B fusion protein Ag11288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 6B
别名
计算分子量 564 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC034535
基因名称 Cytokeratin 6B
Gene ID (NCBI) 3854
RRIDAB_2134318
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04259
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.