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  • Featured Product
  • KD/KO Validated

Cytokeratin 6B Polyclonal antibody

Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 17391-1-AP

Print datasheet

Synonyms

KRT6B, Cytokeratin-6B, Cytokeratin,Keratin, CK-6B, CK6B

验证数据展示

  • WB analysis of mouse skin using 17391-1-AP

    WB analysis of mouse skin using 17391-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17391-1-AP

    IF Staining of HeLa using 17391-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 17391-1-AP

    IHC staining of human lung cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IHC staining of human lung cancer using 17391-1-AP

    IHC staining of human lung cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • FC experiment of HeLa using 17391-1-AP

    FC experiment of HeLa using 17391-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse skin tissue, A431 cells, rat skin tissue
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human tonsil tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1600-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17391-1-AP targets Cytokeratin 6B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Cytokeratin 6B fusion protein Ag11288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 6B
Synonyms KRT6B, Cytokeratin-6B, Cytokeratin,Keratin, CK-6B, CK6B
Calculated Molecular Weight 564 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
RRIDAB_2134318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
IHC protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
IF protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
FC protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Invest Dermatol

Frizzled6 deficiency disrupts the differentiation process of nail development.

Authors - Cui Chang-Yi CY
View Article
humanWB

J Transl Med

Bladder cancer-derived exosomal KRT6B promotes invasion and metastasis by inducing EMT and regulating the immune microenvironment.

Authors - Qiang Song
  • KD Validated
View Article