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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 6B Polyclonal antibody

Cytokeratin 6B Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17391

Print datasheet

Synonyms

CK 6B, CK6B, Cytokeratin 6B, K6B, keratin 6B, KRT6B, KRTL1, PC2, Type II keratin Kb10

验证数据展示

  • IF Staining of HeLa using CL488-17391

    IF Staining of HeLa using CL488-17391

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 6B antibody (CL488-17391) at dilution of 1:200.

  • FC experiment of HeLa using CL488-17391

    FC experiment of HeLa using CL488-17391

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Cytokeratin 6B (CL488-17391) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17391-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17391 targets Cytokeratin 6B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 6B fusion protein Ag11288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 6B
Synonyms CK 6B, CK6B, Cytokeratin 6B, K6B, keratin 6B, KRT6B, KRTL1, PC2, Type II keratin Kb10
Calculated Molecular Weight 564 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC034535
Gene Symbol Cytokeratin 6B
Gene ID (NCBI) 3854
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04259
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 6B antibody CL488-17391Download protocol
FC protocol for CL Plus 488 Cytokeratin 6B antibody CL488-17391Download protocol
Standard Protocols
Click here to view our Standard Protocols