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Cytokeratin 8 Monoclonal antibody

Cytokeratin 8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2H5

Cat no : 68627-1-Ig

Print datasheet

Synonyms

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

验证数据展示

  • WB analysis using 68627-1-Ig

    WB analysis using 68627-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human tonsillitis using 68627-1-Ig

    IHC staining of human tonsillitis using 68627-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included

经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, rat colon tissue, mouse colon tissue, T-47D cells, SK-BR-3 cells, SW480 cells, HT-29 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68627-1-Ig targets Cytokeratin 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 8 fusion protein Ag0488 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 8
Synonyms KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8
Calculated Molecular Weight 54 kDa
Observed Molecular Weight50-52 kDa
GenBank Accession NumberBC000654
Gene Symbol Cytokeratin 8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 68627-1-IgDownload protocol
IHC protocol for Cytokeratin 8 antibody 68627-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols