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Cytokeratin 8 Monoclonal antibody, PBS Only

Cytokeratin 8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2H5

Cat no : 68627-1-PBS

Print datasheet

Synonyms

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

验证数据展示

  • WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

    WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

    IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CK8 antibody included
Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.

产品信息

68627-1-PBS targets Cytokeratin 8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 8 fusion protein Ag0488 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 8
Synonyms KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8
Calculated Molecular Weight 54 kDa
Observed Molecular Weight50-52 kDa
GenBank Accession NumberBC000654
Gene Symbol Cytokeratin 8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05787
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.